Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting

During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) naphthalene-acetic (NAA) were added to basal Murashige Skoog (1962) MS medium. As compared the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted highest up 26 shoots. Enhancement cytokinin concentrations increased shoot number (and case BAP, peroxidase activity) but decreased plant height parameters. Regarding root production, both auxins definitely beneficial (0.2 NAA than 7.5 roots higher auxin efficiently stimulate elongation); however, KIN had similar effects. After a three-month duration time acclimatization, we observed that which previously cultured containing certain (KIN doses 0.1 MT) or greater survival, moreover, as negative after-effect, reduced survived specimens.